General Cell Based Assays
Meso Scale Discovery R-PLEX Mouse MMP-3 total ASY
The Meso Scale Discovery R-PLEX Mouse MMP-3 Total Assay measures total MMP-3 in mouse samples like serum plasma and cell culture supernatants It uses MSD s electrochemiluminescent detection for high sensitivity wide dynamic range and low sample volume The assay can be multiplexed with other R-PLEX assays The kit includes a 96-well plate with capture antibodies detection antibodies calibrators and controls to quantify MMP-3 aiding in the analysis of its role in extracellular matrix remodeling
Eurofins Discoverx PathHunter eXpress CXCR1 (IL8RA) U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0226E3CP0M)
The PathHunter CXCR1 (IL8RA) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL8/IL8) based activation of CXCR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR1 and EA-tagged β-Arrestin-2. Activation of CXCR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical NADP+ (hydrate), Purity: ≥90%, 250 mg
The oxidized form of NADPH; serves as a cofactor in various biological reactions
Eurofins Discoverx PathHunter Human IL-15 (IL-15R (IL2RB/IL2RG)) Dimerization Cell-Based Assay Kit (U2OS)
The PathHunter Human IL-15 (IL-15R (IL2RB/IL2RG)) Stable Cell Line Dimerization Assay (U2OS) measures ligand-induced dimerization of IL2RB/IL2RG. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Enzo Life Sciences FLUOR DE LYS-Green SIRT5 fluorometric drug discovery assay kit (100 tests)
The FLUOR DE LYS Green SIRT5 Fluorometric Drug Discovery Kit is a complete assay system designed to measure the lysyl desuccinylase activity of the recombinant human SIRT5 included in the kit. A black 96-well microplate is packaged with the kit, but it should be noted that reagents of the FLUOR DE LYS system have also been successfully employed in other formats. The Green SIRT5 Fluorescent Activity Assay is based on the unique FLUOR DE LYS-Succinyl GreenSubstrate/Developer combination. The FLUOR DE LYS-Succinyl GreenSubstrate, which comprises a lysine residue, Nε-succinylated on its side-chains, is first incubated with human recombinant SIRT5 together with the cosubstrate NAD+. Desuccinylation of FLUOR DE LYS-Succinyl Green sensitizes it so that, in the second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Alternative name: Green sirtuin 5 fluorescent assay kit. Applications: Fluorescent detection, HTS Cell-based assays, Activity assay.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, 96 Assay Points
HTRF Human & Mouse Phospho-VASP (Ser157) Detection Kit, 96 Assay Points
American Research Products Inc HOXB2 Transcription Factor Activity Assay
HOXB2 Transcription Factor Activity Assay
Eurofins Discoverx PathHunter eXpress PTGFR Total Internalization Assay, 2pl Kit. (93-0872E3CP0M)
The PathHunter PTGFR Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of PTGFR via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged PTGFR and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the PTGFR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR162 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0383E2ACP1M)
The PathHunter GPR162 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR162 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR162 and EA-tagged β-Arrestin-2. Activation of GPR162 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical NFAT Inhibitor, Purity: ≥95%, CAS: 249537-73-3, 10 mg
A peptide inhibitor of NFAT; binds to the NFAT recognition site on calcineurin (IC50 = 25 pM) but does not inhibit calcineurin phosphatase activity at 100 µM; inhibits the dephosphorylation of NFAT in HEK293T lysates expressing NFAT1, -2, or -4; inhibits NFAT activation in RAW 264.7 macrophages, EA.hy926 endothelial cells, and isolated mouse thoracic aorta VSMCs (IC50s = 29.1, 30.2, and 45.7 µM, respectively, in reporter assays)
Eurofins Discoverx PathHunter eXpress KDR/KDR Dimerization Assay, 10-Plate Kit. (93-0996E1CP0L)
The PathHunter KDR/KDR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. VEGF-121) induced dimerization of KDR/KDR receptors. This product uses EFC technology. The cell line included overexpresses KDR target protein fused to PK alongside KDR target protein fused to EA. Upon ligand-induced dimerization of KDR/KDR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mCXCR7 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0689E3MCP5L)
The PathHunter mCXCR7 (CMKOR1) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL12/SDF-1α) based activation of mCXCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR7 and EA-tagged β-Arrestin-2. Activation of mCXCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mP2RY12 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0613E2MCP2M)
The PathHunter mP2RY12 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. ATP) based activation of mP2RY12 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mP2RY12 and EA-tagged β-Arrestin-2. Activation of mP2RY12 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human IFN beta (IFNAR1/IFNAR2) Dimerization Cell-Based Assay Kit (HEK 293)
The PathHunter Human IFN beta (IFNAR1/IFNAR2) Stable Cell Line Dimerization Assay (HEK 293) measures ligand-induced dimerization of IFNAR1/IFNAR2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human SSTR5 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human SSTR5 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure SSTR5 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.